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Table 1 Mutations in dPPCS affect egg chamber development, stage 10–11 F-actin organization & cytoplasmic dumping

From: Drosophila phosphopantothenoylcysteine synthetase is required for tissue morphogenesis during oogenesis

  % of egg chambers
  wild-type dPPCS 1/1 P[dPPCS];dPPCS 1/1
Nurse cells trapped inside the oocyte 0.0 (n = 100) 20.9 (n = 67) 1.8 (n = 56)
F-actin clumps in ooplasm 3.0 (n = 100) 50.1 (n = 53) 7.0 (n = 57)
Aberrant F-actin in nurse cells 0.0 (n = 100) 92.2 (n = 51) 29.5 (n = 61)
Nurse cells plugging ring canals 0.0 (n = 100) 71.0 (n = 62) 15.5 (n = 58)
Oocytes with disorganized subcortical F-actin 0.0 (n = 100) 43.7 (n = 55) 0.0 (n = 53)
Oocyte nuclei with F-actin fibers 0.0 (n = 100) 18.8 (n = 48) 0.0 (n = 46)
  1. Nurse cells were stained with DAPI to detect DNA and labeled with rhodamin-phalloidin to visualize the F-actin network.
  2. P[dPPCS] is a FLAG-tagged dPPCS cDNA under control of a ubiquitin promoter.