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Table 1 Mutations in dPPCS affect egg chamber development, stage 10–11 F-actin organization & cytoplasmic dumping

From: Drosophila phosphopantothenoylcysteine synthetase is required for tissue morphogenesis during oogenesis

 

% of egg chambers

 

wild-type

dPPCS 1/1

P[dPPCS];dPPCS 1/1

Nurse cells trapped inside the oocyte

0.0 (n = 100)

20.9 (n = 67)

1.8 (n = 56)

F-actin clumps in ooplasm

3.0 (n = 100)

50.1 (n = 53)

7.0 (n = 57)

Aberrant F-actin in nurse cells

0.0 (n = 100)

92.2 (n = 51)

29.5 (n = 61)

Nurse cells plugging ring canals

0.0 (n = 100)

71.0 (n = 62)

15.5 (n = 58)

Oocytes with disorganized subcortical F-actin

0.0 (n = 100)

43.7 (n = 55)

0.0 (n = 53)

Oocyte nuclei with F-actin fibers

0.0 (n = 100)

18.8 (n = 48)

0.0 (n = 46)

  1. Nurse cells were stained with DAPI to detect DNA and labeled with rhodamin-phalloidin to visualize the F-actin network.
  2. P[dPPCS] is a FLAG-tagged dPPCS cDNA under control of a ubiquitin promoter.