Table 1 The GP parameters and limits for different filtering conditions in GP.
From: Advanced spot quality analysis in two-colour microarray experiments
| Â | Â | Filtering conditions | |||
|---|---|---|---|---|---|
| Â | Parameter | Strong | Medium | Weak | Standard |
1 | Dia. | >= 120 | >= 100 | >= 70 | NR |
2 | % > B635+2SD | > 98 | > 95 | > 66 | > 55* |
3 | % > B532+2SD | > 98 | > 95 | > 66 | > 55 |
4 | F635% Sat. | < 1 | < 5 | < 10 | < 2–3 |
5 | F532% Sat. | < 1 | < 5 | < 10 | < 2–3 |
6 | Rgn Ratio (635/532) | > 0 | > 0 | > 0 | NR |
7 | Rgn R2 (635/532) | > 0.8 | > 0.6 | > 0.4 | > 0.5 |
8 | Circularity | > 85 | > 80 | > 50 | > 80 |
9 | F635 Median – B635 | > 600 | > 400 | > 200 | NR |
10 | F532 Median – B532 | > 600 | > 400 | > 200 | NR |
11 | SNR 635 | > 10 | > 5 | > 2 | > 3 |
12 | SNR 532 | > 10 | > 5 | > 2 | > 3 |
13 | Flags | >= 0 | >= 0 | >= 0 | >= 0 |