Figure 2

White adipose tissue enrichment of Tmem182 and dysregulation in ob/ob adipose tissues. (A) Tmem182 transcript expression in various murine tissues. RNA was harvested from the indicated murine tissues and Tmem182 transcript level was analyzed by real-time PCR as described in Methods. Values were normalized to Gapdh expression level and transcript expression in lung was set to a value of 1. Statistical analysis was carried out with one-way ANOVA followed by Tukey post-hoc test. ** indicates p < 0.001 for SC WAT vs. all other tissues. * indicates p < 0.01 for muscle, heart, and lung vs. all other tissues. (B) Tmem182 transcript expression in wild type brown and white adipose tissues and (C) Expression of Tmem182 transcript in wild type (wt) vs. ob/ob white and brown adipose tissues. RNA was harvested from subcutaneous (SC) WAT, epididymal (EP) WAT and brown adipose tissue (BAT) of wild type (wt) and ob/ob (ob) mice and real-time PCR for Tmem182 transcript was performed as described in Methods. Values were normalized to Gapdh transcript expression level. Transcript expression in BAT was set to a value of 1 for (B) and wt was set to a value of 1 for (C). Statistical analysis was carried out with single-factor ANOVA. For (B), * indicates p < 0.001 for SC, EP, and RP vs. BAT. For (C), * indicates p < 0.001 for ob BAT vs. wt BAT and for ob SC vs. wt SC.