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Table 2 Primers and PCR conditions used for mutation screening of the coding exons of CHD5.

From: Mutation screening of CHD5 in melanoma-prone families linked to 1p36 revealed no deleterious coding or splice site changes

Exon Primer (forward/reverse) Annealing Product size (bp)
2 5'-CTCTCACTTCACTGGGTTTG-3'
5'-GAAACCCTCAAACTCCAAGG-3'
58 393
3 5'-CTCTGATGATGAGTGGAGTG-3'
5'-AACATACAGGCAAGAGGCTCAG-3'
58 394
4 5'-TTTCCTAGGGTGGGTGAGAATG-3'
5'-TTGCTCAGTCGGTCTGACAGAG-3'
58 400
5 5'-CTCTCTAATCAGGAACCTGG-3'
5'-GGCTTCTCCTATAGGGTCTGAAAG-3'
58 441
6 5'-CCCTTTCCTTATTGGGTAACCG-3'
5'-GCCCCAGCTAGTTTGTAATG-3'
58 335
7 5'-GAATCACAGAGAGCACTGTG-3'
5'-TCCTTGTTCTTTCCTTACTGGG-3'
58 491
8 5'-ACATCTACTCTGTGCCTGTCTG-3'
5'-GCATTCTGCCCCCAAATGAG-3'
58 347
9 5'-TGTAGGGGAGGGAGGGAGTC-3'
5'-TTTTGAGGAGGGCAGGCCTTC-3'
67 387
10 5'-CGTGGTACTGTTCTGACTTG-3'
5'-CTCAGTCAGAGGCGCTTCAG-3'
58 435
11 5'-CTGAAGCGCCTCTGACTGAG-3'
5'-TGCGCTGCACCCATTTTACAG-3'
58 459
12 & 13 5'-TGCCCTTCATCAAACCTGTG-3'
5'-CCTGCACATTCAAGTCTGAG-3'
58 472
14 5'-GAATTGCATGTGCAAAGGCCTG-3'
5'-CGCGTTCCCAGTTGATGATG-3'
58 397
15 5'-CCTTTACTCCCTCTACAAGG-3'
5'-ACCCGTGGTCCCTGAACTAG-3'
58 373
16 & 17 5'-TTGGCTCTTTGTCTCCTGGG-3'
5'-CAGGATGGGCTATTGATCCG-3'
58 504
18 5'-TGAGACGATATCCAGGGCAATG-3'
5'-AAAGCATTAGCCGAGACCTCAG-3'
58 431
19 5'-GGTCTCTCTGTAAATGGGTGCTTG-3'
5'-AAACCTACCATGACAGCCACAG-3'
58 396
20 5'-CAGCACTTGTCTGTTCCCTG-3'
5'-ACACAGTCACATGACCCACATG-3'
58 357
21 5'-GGGTGAGGTTGGAAGCTTTG-3'
5'-GTCTTTAGCTGTTTGTGAGGCTG-3'
58 419
22 5'-TTGCTGCAGTTCCTTCTCTCTG-3'
5'-ATGGCAAGAAGGGCATGAAG-3'
58 311
23 5'-TCCATGCTCTTGAGCTCATG-3'
5'-AGAAGAGAGGCTGTGTGTTG-3'
58 429
24 5'-AACACACAGCCTCTCTTCTG-3'
5'-AGGAAACTGCGCTGTAACAG-3'
58 393
25 5'-GCAGTTTCCTCTGTCTGGTCAC-3'
5'-TCACAGCTTGGAGGGCTGGCTG-3'
58 442
26 5'-GGCCATCCCATTAATCCTTG-3'
5'-GATGCCCTGACAGAATCCTG-3'
58 370
27 & 28 5'-CCTGGGCATGCTTGAACTTG-3'
5'-CAGACCAAGTTCTGTCCAAG-3'
58 522
29 5'-CTTGGACAGAACTTGGTCTG-3'
5'-CATCCTGGCGGAAGCAAATG-3'
58 419
30 5'-AGCCCTCTCAGAGGGTTCTTG-3'
5'-ACAGCACCAGGAGCCCAGGCAG-3'
58 249
31 5'-CAAGCCTGTGACACTTTCAG-3'
5'-GATTGTGGGTTAGACTAGGG-3'
58 351
32 5'-CCTCACTTTGGTCTTACTGG-3'
5'-CGATTCAGAGCCCCGAAAAG-3'
58 440
33 5'-CTTTTCGGGGCTCTGAATCG-3'
5'-CTCTCTGCCAGGGAGAAATG-3'
58 391
34 5'-TTGATGGATAGGGTTCCATGGG-3'
5'-AGGGCCTAGAGGTATGCAAAG-3'
58 488
35 5'-GCTTGTTTAAGACCCTTCTGGG-3'
5'-TAACCACTGGTCTAGACTCCTG-3'
58 363
36 5'-TTAGCCACCCTGGAACACTG-3'
5'-GGAAGATTGAGGAAGAACGAGG-3'
58 352
37 5'-TCCCTGAGCTGCCTCCCCCTAC-3'
5'-AGGGTCCTCCTGACACCGTC-3'
58 267
38 5'-TACGTCCTCTTTCCCTCCTTTCTG-3'
5'-TGCCCTCATCTACAGCCAAG-3'
58 393
39 5'-CATCCTTCCACTCCTCCATC-3'
5'-AGCTTCACAGGTGGTCTCAG-3'
58 325
40 & 41 5'-GTGCCCCTGGGTGGAGGCTG-3'
5'-ACTGTGGCCAGGCCTGGTTTG-3'
58 410
  1. Exons 12 & 13, 16 & 17, 27 & 28, and 40 & 41 were amplified as a single amplicon.
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