Table 2 Primers and PCR conditions used for mutation screening of the coding exons of CHD5.

2

5'-CTCTCACTTCACTGGGTTTG-3'

5'-GAAACCCTCAAACTCCAAGG-3'

3

5'-CTCTGATGATGAGTGGAGTG-3'

5'-AACATACAGGCAAGAGGCTCAG-3'

4

5'-TTTCCTAGGGTGGGTGAGAATG-3'

5'-TTGCTCAGTCGGTCTGACAGAG-3'

5

5'-CTCTCTAATCAGGAACCTGG-3'

5'-GGCTTCTCCTATAGGGTCTGAAAG-3'

6

5'-CCCTTTCCTTATTGGGTAACCG-3'

5'-GCCCCAGCTAGTTTGTAATG-3'

7

5'-GAATCACAGAGAGCACTGTG-3'

5'-TCCTTGTTCTTTCCTTACTGGG-3'

8

5'-ACATCTACTCTGTGCCTGTCTG-3'

5'-GCATTCTGCCCCCAAATGAG-3'

9

5'-TGTAGGGGAGGGAGGGAGTC-3'

5'-TTTTGAGGAGGGCAGGCCTTC-3'

10

5'-CGTGGTACTGTTCTGACTTG-3'

5'-CTCAGTCAGAGGCGCTTCAG-3'

11

5'-CTGAAGCGCCTCTGACTGAG-3'

5'-TGCGCTGCACCCATTTTACAG-3'

12 & 13

5'-TGCCCTTCATCAAACCTGTG-3'

5'-CCTGCACATTCAAGTCTGAG-3'

14

5'-GAATTGCATGTGCAAAGGCCTG-3'

5'-CGCGTTCCCAGTTGATGATG-3'

15

5'-CCTTTACTCCCTCTACAAGG-3'

5'-ACCCGTGGTCCCTGAACTAG-3'

16 & 17

5'-TTGGCTCTTTGTCTCCTGGG-3'

5'-CAGGATGGGCTATTGATCCG-3'

18

5'-TGAGACGATATCCAGGGCAATG-3'

5'-AAAGCATTAGCCGAGACCTCAG-3'

19

5'-GGTCTCTCTGTAAATGGGTGCTTG-3'

5'-AAACCTACCATGACAGCCACAG-3'

20

5'-CAGCACTTGTCTGTTCCCTG-3'

5'-ACACAGTCACATGACCCACATG-3'

21

5'-GGGTGAGGTTGGAAGCTTTG-3'

5'-GTCTTTAGCTGTTTGTGAGGCTG-3'

22

5'-TTGCTGCAGTTCCTTCTCTCTG-3'

5'-ATGGCAAGAAGGGCATGAAG-3'

23

5'-TCCATGCTCTTGAGCTCATG-3'

5'-AGAAGAGAGGCTGTGTGTTG-3'

24

5'-AACACACAGCCTCTCTTCTG-3'

5'-AGGAAACTGCGCTGTAACAG-3'

25

5'-GCAGTTTCCTCTGTCTGGTCAC-3'

5'-TCACAGCTTGGAGGGCTGGCTG-3'

26

5'-GGCCATCCCATTAATCCTTG-3'

5'-GATGCCCTGACAGAATCCTG-3'

27 & 28

5'-CCTGGGCATGCTTGAACTTG-3'

5'-CAGACCAAGTTCTGTCCAAG-3'

29

5'-CTTGGACAGAACTTGGTCTG-3'

5'-CATCCTGGCGGAAGCAAATG-3'

30

5'-AGCCCTCTCAGAGGGTTCTTG-3'

5'-ACAGCACCAGGAGCCCAGGCAG-3'

31

5'-CAAGCCTGTGACACTTTCAG-3'

5'-GATTGTGGGTTAGACTAGGG-3'

32

5'-CCTCACTTTGGTCTTACTGG-3'

5'-CGATTCAGAGCCCCGAAAAG-3'

33

5'-CTTTTCGGGGCTCTGAATCG-3'

5'-CTCTCTGCCAGGGAGAAATG-3'

34

5'-TTGATGGATAGGGTTCCATGGG-3'

5'-AGGGCCTAGAGGTATGCAAAG-3'

35

5'-GCTTGTTTAAGACCCTTCTGGG-3'

5'-TAACCACTGGTCTAGACTCCTG-3'

36

5'-TTAGCCACCCTGGAACACTG-3'

5'-GGAAGATTGAGGAAGAACGAGG-3'

37

5'-TCCCTGAGCTGCCTCCCCCTAC-3'

5'-AGGGTCCTCCTGACACCGTC-3'

38

5'-TACGTCCTCTTTCCCTCCTTTCTG-3'

5'-TGCCCTCATCTACAGCCAAG-3'

39

5'-CATCCTTCCACTCCTCCATC-3'

5'-AGCTTCACAGGTGGTCTCAG-3'

40 & 41

5'-GTGCCCCTGGGTGGAGGCTG-3'

5'-ACTGTGGCCAGGCCTGGTTTG-3'