PCR to detect gDNA, RNA electropherograms and RT-qPCR on Arabidopsis seeds and leaves. (A) PCR products after 35 cycles with β-tubulin specific oligonucleotides on DNase treated total RNA isolated from dry (0), stratified (S) and germinating seeds (6 to 48 hours); (-): no template; (+): gDNA; (M): molecular marker. (B) qPCR with AtDOF31 specific oligos on cDNAs prepared from total RNA samples isolated from leaves of wild-type (Col-0) or transgenic plants overexpressing the AtDOF31 gene (lines 1 to 6). Expression levels relative to ubiquitin (UBQ; At5g25760) are shown and numbers in italic inside the graph indicates fold differences with Col-0. (C) Electropherograms of 500 ng total RNA samples from seeds and siliques (Agilent 2100 Bioanalyzer). Electrophoretic RNA migration time and fluorescence values correlate with RNA size and quantity, respectively, and are calculated by comparison to a RNA ladder (Agilent Technologies, Palo Alto, CA, USA).