Figure 1
From: Ablation of prion protein immunoreactivity by heating in saturated calcium hydroxide
Western blot of non-treated brain homogenates. Representative western blots of scrapie-positive and scrapie-negative sheep brain homogenates. Lane 1-Molecular weight marker; lane 2-Scrapie-positive brain without proteinase K digestion; lane 3-Scrapie-positive brain treated with proteinase K; lane 4-Blank; lane 5-Scrapie-negative brain without proteinase K digestion; lane 6-scrapie-negative brain with proteinase K digestion. Only the scrapie-positive brain homogenate exhibits proteinase K resistance.