Figure 2
From: Ablation of prion protein immunoreactivity by heating in saturated calcium hydroxide
Western blot after calcium hydroxide treatment. Western blot analysis of calcium hydroxide treatment of scrapie-negative (A) and scrapie-positive (B) sheep brain homogenate. For both, Lane 1-molecular weight marker; lane 2–0 minutes; lane 3–10 minutes; lane 4–20 minutes; lane 5–30 minutes; lane 6–60 minutes; lane 7–120 minutes; lane 8–240 minutes. PrP immunoreactivity is abolished in scrapie-positive samples after 10 minutes treatment with calcium hydroxide.