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Table 2 Effect of Z-VAD-FMK on the cytotoxicity induced by HCR-NTPase and E114A-HCR-NTPase in SH-SY5Y

From: On the cytotoxicity of HCR-NTPase in the neuroblastoma cell line SH-SY5Y

Treatment

Fluorescent cells

GFP (control)

100 (20)

GFP + Z-VAD-FMK

93 (15)

GFP + HCR-NTPase

4 (4)

GFP + HCR-NTPase + Z-VAD-FMK

91 (21)

GFP + E114A-HCR-NTPase

79 (12)

GFP + E114A-HCR-NTPase + Z-VAD-FMK

95 (17)

  1. SH-SY5Y cells were transfected with plasmids containing green fluorescent protein (GFP), HCR-NTPase or E114A-HCR-NTPase. The percentage of total cells normalised to 100 for cells only treated with GFP (control) as microscopically judged by green fluorescence are given with and without adding the caspase inhibitor Z-VAD-FMK. In parenthesis the standard deviations are stated. Three experiments were performed independently.