Treatment
|
Fluorescent cells
|
---|
GFP (control)
|
100 (20)
|
GFP + Z-VAD-FMK
|
93 (15)
|
GFP + HCR-NTPase
|
4 (4)
|
GFP + HCR-NTPase + Z-VAD-FMK
|
91 (21)
|
GFP + E114A-HCR-NTPase
|
79 (12)
|
GFP + E114A-HCR-NTPase + Z-VAD-FMK
|
95 (17)
|
- SH-SY5Y cells were transfected with plasmids containing green fluorescent protein (GFP), HCR-NTPase or E114A-HCR-NTPase. The percentage of total cells normalised to 100 for cells only treated with GFP (control) as microscopically judged by green fluorescence are given with and without adding the caspase inhibitor Z-VAD-FMK. In parenthesis the standard deviations are stated. Three experiments were performed independently.