Localization of Kinesin-5-like proteins (A-H) and morphology of short bipolar and multipolar spindles (I, J). Microtubules are in green in all images. Kinesin-5-like proteins are in red in A-C, E, F, H. Condensed chromatin is in blue in I and J. (A) 17-h old zygote in interphase. (B) Thallus cell of a 2-day old embryo entering prophase. (C-H) Two metaphase spindles are shown; anti-Kinesin-5 signal (C, F), microtubules (D, G), and merged images (E, H). In F and H, anti-Kinesin-5 signal was collected with increased amplifier gain and adjusted for contrast and brightness to enhance visualization of the weak signal; microtubule signal was not enhanced. Arrows indicate Kinesin-5-like motors colocalizing with microtubules in B and E, at the spindles poles and midzone in C and F, respectively. (I, J) Effect of monastrol treatment on metaphase spindles. Condensed chromatin is associated with short bipolar spindles in I and multipolar spindles in J following treatment with monastrol. Secondary antibodies commonly stick to zygotes' extracellular adhesive and is seen in A. Scale bars equal 10 μm.