Assessment of DNA extracted from FTA®cards for use on the Illumina iSelect BeadChip

BMC Research Notes

Table 2 Genotype call and concordance percentage rates for DNA samples extracted from FTA cards, by sample type.

	Standard	Blood FTA	Blood FTA Elute	Nasal Swab FTA Elute
Call Rate %	98.86	99.04 (0.07)	98.44 (0.44)	95.78 (0.25)
Concordance Rate %	99.006	99.817 (0.40)	99.786 (0.40)	99.573 (0.43)
Alternative Homozygous Rate %	0.006	0.000 (0.40)	0.000 (0.40)	0.002 (0.43)
Homozygous vs. Heterozygous Rate %	0.988	0.183 (0.40)	0.214 (0.40)	0.425 (0.43)

Two sample t-tests assuming equal within-treatment variances, the number in parenthesis is the P-value corresponding to the comparison of that sample to the standard. Two samples from each FTA type were compared to 7,737 samples extracted by PCI for call rate and to 35 samples extracted by PCI for which dual aliquots were genotyped on the Bovine SNP50 BeadChip for concordance, alternative homozygous, and homozygous vs. heterozygous rate.

ISSN: 1756-0500