Skip to main content
Figure 1 | BMC Research Notes

Figure 1

From: Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays

Figure 1

Determining the optimum primer concentrations. (A) Distribution of the relative Cq differences (the difference between the primer combination showing the highest Cq value and the primer combination showing the lowest Cq value for each assay) obtained for each of the 60 RT-qPCR assays. (B) Distribution of the optimal primer concentration combinations for each of the 60 assays. The primer concentration combinations are given in nmol/L for the forward and the reverse primers respectively.

Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us