IL-4 DC-T inhibit the proliferation of antigen-specific T cells in a dose-dependent manner. Different numbers of IL-4 DC-T which were in contact with IL-4 DC during the first culture (without transwell) were added to the proliferation assay containing freshly isolated P1-specific T cells and P1-loaded S-DC. The IL-4 DC-T inhibited their restimulation in a cell number dependent manner (A). Antigen-specific T cells which were not in contact with IL-4 DC during the first culture (transwell experiment) had no inhibitory effect (B). The results (mean ± standard deviation) are representative for 3 different experiments.