From: Ultraviolet-ozone treatment reduces levels of disease-associated prion protein and prion infectivity
Inoculum | Positive/Total Animals | Onset of Clinical Symptoms (dpi) | Estimated Titer (ID50 per 50 μL dosage) | Approximate Reduction in Titer |
---|---|---|---|---|
Dilutions of Starting Material | ||||
HY BH (10% w/v) | 4/4 | 67 ± 0* | 106-107 | Not applicable |
HY BH (102 dilution factor) | 8/8 | 86 ± 0* | 104-105 | 102 |
HY BH (104 dilution factor) | 8/8 | 107 ± 0* | 102-103 | 104 |
HY BH (106 dilution factor) | 2/8 | 141, 156†,‡ | 0–101 | 106 |
PBS | 0/4 | >365‡ | 0 | Not applicable |
UV-ozone Treated Material | ||||
HY BH (10% w/v) ashed 4 weeks | 4/4 | 120 ± 10* | 101-102 | 105 |
HY BH (10% w/v) ashed 8 weeks | 5/7 | 127, 136, 136, 136, 141†,‡ | 1–101 | 106 |