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Table 1 UV-ozonation decreases infectious TSE titer and increases disease incubation.

From: Ultraviolet-ozone treatment reduces levels of disease-associated prion protein and prion infectivity

Inoculum

Positive/Total Animals

Onset of Clinical Symptoms (dpi)

Estimated Titer (ID50 per 50 μL dosage)

Approximate Reduction in Titer

Dilutions of Starting Material

    

HY BH (10% w/v)

4/4

67 ± 0*

106-107

Not applicable

HY BH (102 dilution factor)

8/8

86 ± 0*

104-105

102

HY BH (104 dilution factor)

8/8

107 ± 0*

102-103

104

HY BH (106 dilution factor)

2/8

141, 156†,‡

0–101

106

PBS

0/4

>365

0

Not applicable

UV-ozone Treated Material

    

HY BH (10% w/v) ashed 4 weeks

4/4

120 ± 10*

101-102

105

HY BH (10% w/v) ashed 8 weeks

5/7

127, 136, 136, 136, 141†,‡

1–101

106

  1. * Mean days post inoculation (dpi) ± SD to onset of clinical symptoms of TSE infection
  2. Number of dpi to onset of clinical TSE symptoms for each clinically-affected animal in the group
  3. Animals showing no clinical signs of TSE infection were sacrificed 365 days post-inoculation