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Table 1 fH binding proteins, binding strength, calculated molecular mass and isoelectric points estimated in 2-DE.

From: Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human

Borrelia species (strain) OspA Serotype MW of fHBP Estimated pIa Human and animal fH binding affinity
     Hb M R G C Ho D Ca
B. burgdorferi s.s. (SKT2) 1 ~26 kDa c 8.0-8.1 +++d +++ - - - - - -
B. afzelii (SKT4) 2 ~15 kDa 4.0-5.2 - +++ - - - - +++ ++
   ~26 kDa 6.8-7.1 +++ ++ - - - - - -
B. garinii (Rio2) 3 - - - - - - - - - -
B. garinii (PBi) 4 ~19 kDa 6.0-7.0 +++ - - - - - - -
   ~28 kDa 5.0-5.3 - +++ - - - - - -
B. garinii (G117) 5 ~26 kDa 5.6-6.2 -e ++ - - - - - -
B. garinii (SKT3) 6 - - - - - - - - - -
B. garinii (T25) 7 ~17 kDa 5.0-5.5 ++ - - - - - - -
B. garinii (CL1) 8 - - - - - - - - - -
B. valaisiana (VS116) NAf ~17 kDa 4.2-5.0 +++ - - - - - +++ -
B. andersonii (21123) NA ~15 kDa 5.5-6.1 + - - - - - - -
   ~17 kDa 8.0-8.8 + - - - - - + -
   ~23 kDa 6.1-6.6 + ++ - - - - - -
   ~26 kDa 8.0-8.3 - ++ - - - - - -
B. lusitaniae (Poti B2) NA - - - - - - - - - -
B. bissettiii (DN127) NA ~25 kDa 8.0-8.5 + - - - - - - -
   ~28 kDa 5.0-5.5 - +++ - - - - - -
   ~40 kDa 6.9-7.8 - + - - - - - -
B. japonica (HO14) NA ~15 kDa 4.8-5.2 - +++ ++ - - - - -
   ~19 kDa 4.5-5.0 - - ++ - - - - -
   ~22 kDa 6.0-6.8 + - - - - - ++ ++
   ~24 kDa 4.5-4.9 - - ++ - - - - -
   ~26 kDa 7.9-8.3 - +++ - - - - - -
B. hermsii (HS1) NA ~20 kDa 8.0-8.3 +++ +++ +++ +++ - - - -
B. parkeri (M3001) NA ~23 kDa 8.0-8.5 ++ - - - - - - -
B. anserina (ES-1) NA - - - - - - - - - -
B. coriaceae (Co53) NA ~40 kDa
~58 kDa
7.0-8.0
5.5-5.7
-
-
+++
+++
-
++
-
-
+++
+++
-
-
-
-
-
-
  1. apI: isoelectric point; bH: human; M: mouse; R: rat; G: guinea pig; C: cattle; Ho: horse; D: dog; Ca: cat; cboldface indicate those proteins identified by the mass spectrophotometry and related analysis in this study;dfH binding strength. +++: high; ++: medium; +: weak; -: negative; eThis protein was positive when purified human fH was used; fNA: not applicable;