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Table 1 SEC evaluation of predicted stabilized or mildly destabilized MICA mutants

From: Size-exclusion chromatography can identify faster-associating protein complexes and evaluate design strategies

MIC Mutant

Design Score

SEC Binding

N69Q_Q108L_Q120I_K154S_T155D

-8.1

+

N69Q_Q120I_K154S_T155D_Y157L

-7.1

-

N69Q_D72F_K154S_T155D

-7.1

+

N69Q_Q120I_K154S_T155D

-6

+

N69Q_D72F_Q108L_K152V_Y157L

-5.9

+

K152V_K154S_T155D_Y157L

-5.8

+

N69Q_K154D

-4.3

+

K154S_T155D

-4

-

K152V_K154S_T155D

-3.9

-

N69Q_D72F_Q108L

-3.9

+

N69Q_D72F

-3.6

+

N69Q

-2.8

+

K152V_Y157L

-2.2

+

K154D

-1.5

+

Q108L

-0.5

+

Wild-type

0

+

D72W

0.3

-

Q120I

0.7

+

Q120I_K154M

0.8

n/a

N69W

1.8

++

N69W_K152E_K154S

4.2

++

N69W_K152E_K154D

4.3

++

K152E_K154M

4.4

+

N69W_D72F_K152E

4.5

++

N69W_D72W_K152E

5.1

+

N69W_K152E

5.5

++

  1. Design scores are listed relative to wild-type. Results of SEC binding assay follow the examples in Figure 2; n/a, not applicable because unbound mutant did not elute from column due to non-specific binding.
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BMC Research Notes

ISSN: 1756-0500

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