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Table 1 SEC evaluation of predicted stabilized or mildly destabilized MICA mutants

From: Size-exclusion chromatography can identify faster-associating protein complexes and evaluate design strategies

MIC Mutant Design Score SEC Binding
N69Q_Q108L_Q120I_K154S_T155D -8.1 +
N69Q_Q120I_K154S_T155D_Y157L -7.1 -
N69Q_D72F_K154S_T155D -7.1 +
N69Q_Q120I_K154S_T155D -6 +
N69Q_D72F_Q108L_K152V_Y157L -5.9 +
K152V_K154S_T155D_Y157L -5.8 +
N69Q_K154D -4.3 +
K154S_T155D -4 -
K152V_K154S_T155D -3.9 -
N69Q_D72F_Q108L -3.9 +
N69Q_D72F -3.6 +
N69Q -2.8 +
K152V_Y157L -2.2 +
K154D -1.5 +
Q108L -0.5 +
Wild-type 0 +
D72W 0.3 -
Q120I 0.7 +
Q120I_K154M 0.8 n/a
N69W 1.8 ++
N69W_K152E_K154S 4.2 ++
N69W_K152E_K154D 4.3 ++
K152E_K154M 4.4 +
N69W_D72F_K152E 4.5 ++
N69W_D72W_K152E 5.1 +
N69W_K152E 5.5 ++
  1. Design scores are listed relative to wild-type. Results of SEC binding assay follow the examples in Figure 2; n/a, not applicable because unbound mutant did not elute from column due to non-specific binding.