Skip to main content

Table 1 Primers used for J. onca cDNA and genomic PCR.

From: The opsin repertoire of Jenynsia onca: a new perspective on gene duplication and divergence in livebearers

Opsin category Primer Name Sequence
SWS1 SWS1Fw1 5'-AACTACATCYTGGTMAACATCTCC-3'
  SWS1Rev2 5'-GAACTGTTTGTTCATGAAGGCG-3'
SWS2 SWS2Fw1 5'-GYACWATTCAATACAAGAARC-3'
  SWS2Rev2 5'-TCTCWGCCTTCTGGGTKGAGGC-3'
  SWS2AFw1 5'-GTCCACCCGAGTCATAGAGC-3'
  SWS2ARev2 5'-GCCCACGGTTGTTGACAAC-3'
RH2 RH2Fw1 5'-AACTTCTAYATCCCGWTGTCC-3'
  RH2Rev1 5'-AGCATGCAGTTACGGACTG-3'
  RH2-2Fw1 5'-CAACAGGACGGGCTGGTGAGG-3'
  RH2-2Rev3 5'-ACCCATTCCAATTGTTGCC-3'
RH1 RH1Fw2 5'-GGAGTCCTTATGAATATCCTCAG-3'
  RH1Rev2 5'-CCTGTTGCTCCATTTATGCAGG-3'
LWS Fw100 5'-GATCCCTTTGAAGGACCAAACT-3'
  Fw1a 5'-TCTTATCAGTCTTCACCAACGG-3'
  RevEnd 5'-TTATGCAGGAGCCACAGAGG-3'
  Rev8 5'-GCCCACCTGTCGGTTCATGAAG-3'