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Table 1 AFLP primer pairs used for map construction

From: A genetic linkage map of Venturia inaequalis, the causal agent of apple scab

AFLP primer pairs Polymorphic bands Markers with distorted segregation from 1:1a Mapped markers
Code Composition Q W Total P 0.05 P < 0.01 P < 0.001 Q W Total
F Mb+C/Ec+AGd 17 13 30 11 11 9 9 10 19
H M+C/E+AT 18 24 42 20 19 12 9 10 19
K M+C/E+TG 19 17 36 15 11 10 13 10 23
L M+G/E+TG 18 19 37 27 26 22 3 8 11
M M+G/E+AG 7 11 18 11 8 6 4 10 14
N M+G/E+AT 25*** e 5 30 17 13 10 6 4 10
O M+A/E+AG 15 8 23 16 15 14 4 6 10
P M+A/E+AT 14* 4 18 13 13 8 11 4 15
R M+T/E+AG 17 17 34 15 13 11 8 10 18
S M+T/E+AT 21 21 42 24 18 15 14 13 27
T M+C/E+AA 8 8 16 12 12 9 1 4 5
V M+C/E+AC 16 14 30 18 9 6 10 13 23
W M+A/E+TG 8 5 13 9 8 7 4 0 4
X M+T/E+TG 14 19 33 18 16 12 8 10 18
Y M+A/E+AA 8 12 20 15 13 11 5 5 10
Z M+T/E+AA 23 12 35 20 19 16 9 7 16
AA M+A/E+AC 16 14 30 16 13 13 12 12 24
BB M+T/E+AC 16 15 31 18 14 9 9 8 17
  Total 280 238 518 295 251 200 139 144 283
  1. Table 1 lists the AFLP primer pairs used for screening apple scab isolates, and segregation and mapping data for AFLP markers generated for the construction of the genetic linkage map of Venturia inaequalis
  2. a: Number of markers with a segregation ratio deviating from the 1:1 ratio based on the exact probability from an one sample binomial test
  3. b: M stands for MseI sequence – GATGAGTCCTGAGTAA (5' to 3')
  4. c: E stands for EcoR1 sequence – AGACTGCGTACCAATTC (5' to 3')
  5. d: FAM was attached to E + AA, E + AC and E + AT; VIC to E + AG and NED to E + TG
  6. e: *, *** indicates that the C0154 isolate contributed significantly more alleles to the progeny than the 01/213 isolate at the level of P = 0.05 and 0.001, respectively