Figure 3

Conservation of a predicted secondary structure in NoV ORF1 sequences. Multiple sequence alignment across 49 Human NoV ORF1 sequences shows a consensus secondary structure from positions 3922 through 3973 of the NoV genome (positions 240 through 291 of the RdRp sequence, relative to strain [EMBL:EU794713]). The structure is shown in the dot-bracket format [16] above the alignment. Each corresponding bracket represents consensus base pairs of the alignment columns beneath. Sequences are color-coded according to consistent and compensatory mutations in the aligned sequences regarding the conserved structure (see figure text box). Pale colors indicate that a base-pair cannot be formed in some sequences of the alignment. The sequence conservation profile is shown in gray bars bellow the alignment. The conserved predicted secondary structure, color-coded according to the different types of base pairs in the corresponding alignment columns, is shown on the right side of the figure.