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Table 1 Occurrence of "common" deletion in human spermatozoa using different primer sets

From: Correlation of the 4977 bp mitochondrial DNA deletion with human sperm dysfunction

    Occurrence of deletion
# Primer set Product size Our dataa Literature data
1 4977F – 4977Rc 380 bp 58.39% (87/149) 53.12% (34/64)b
2 4977F – 4977R 454 bp 42.95% (64/149) 56.55% (15/27)c
3 4977F – 4977RK2 524 bp 34.89% (52/149) 37.5% (15/40)d
4 4977F – 4977RK3 719 bp 17.45% (26/149) Not available
  1. a PCR in 45% percoll fraction
  2. b PCR in non percoll fractionated sperm [11].
  3. c nested PCR with reverse primer MT13 [see supplementary Table 1 of Additional file] in 45% percoll fraction [12].
  4. d PCR in 50% percoll fraction [10].
  5. Chi-square test between our data and the data from the literature gives P = 0.477 for primer set #1, P = 0.226 for primer set #2, and P = 0.760 for primer set #3