Whole blood of eight patients with active TB, and seven healthy unexposed controls was stimulated 20 hours with either saline or M. tuberculosis specific antigens. Biomarker production was measured in plasma supernatant by ELISA (IFN-γ) and multiplex technology (IP-10, MCP-3, MCP-2, IL-1RA and MCP-1). Antigen-dependent production represent the antigen stimulated sample subtracted the un-stimulated sample. Straight lines represent median values. Differences between healthy controls and patients were all significant p < 0.0001 (Kruskal-Wallis test).