Figure 2

Histological analysis of BUB1Δ/+ and BUB1Δ/Δ embryos. (A) Images of BUB1^Δ/+ and BUB1^Δ/Δ embryos harvested 3 days after tamoxifen injection. Scale bar, 5 mm. (B) Images of same embryos as in A, after removal of the placenta and yolk sac. Scale bar, 5 mm. (C) Sagittal section of the same BUB1^Δ/Δ embryo as in A and B, stained with haematoxylin-eosin, showing signs of extensive haemorrhaging. (D) Enlargements of regions marked in C, showing haemorrhaging (arrows) in the brain area (panel i), the spinal cord (panel ii) and the body cavity (panel iii). Scale bars, 0.5 mm.(E) Embryos were harvested at 2-5 days after tamoxifen injection, fixed, then sections prepared and stained with haematoxylin-eosin. Scale bar, 1 mm.