SDHB MS-HRM and MSP analysis in neuroblastoma cell lines. A. SDHB MS-HRM of the SK-N-SH neuroblastoma cell line; the early melting shows that SDHB is clearly unmethylated in the SK-N-SH cell line isolate used by us (the curves for each sample represents the mean value of duplicate samples). B. Four bisulfite modified neuroblastoma DNA (IMR32, SH-SY5Y, Be(2)c and SK-N-SH) were amplified using specific SDHB methylated (M) and unmethylated (U) MSP primer pairs. Fully methylated DNA (100% methyl) and fully unmethylated DNA (WGA) were used as controls. The no template control (NTC) was also included as a negative control for both methylated and unmethylated PCR.