Skip to main content

Table 4 Primers used for amplification of NBS sequences

From: TIR-NBS-LRR genes are rare in monocots: evidence from diverse monocot orders

Specificity (abbreviation)

Amino acid sequence

Degenerate primer (5'-3')

Conserved P-loop/Kinase-1a (V)

GVGKTT

GGIGTIGGIAARACIAC

P-loop/Kinase-1a TIR (I)

GIGKTT

GGIATHGGIAARACIAC

RNBS-D TIR primer 1L (T1L)

FLHIACFF

RAARAARCAIGCDATRTGIARRAA

RNBS-D TIR primer 1 (T1)

FLHIAC

CAIGCDATRTGIARRAA

RNBS-D TIR primer 2 (T2)

FLHIAC

CANGCDATRTGAARRAA

RNBS-D TIR primer 3 (T3)

FLHIAC

CANGCDATRTGCARRAA

RNBS-D TIR primer 4 (T4)

FLHIAC

CANGCDATRTGGARRAA

RNBS-D TIR primer 5 (T5)

FLHIAC

CANGCDATRTGTARRAA

RNBS-D non-TIR primer 1 (nT1)

CFLYCALFPED

CYTCIGGRAAIARIGCRCARTAIARRAARC

RNBS-D non-TIR primer 2 (nT2)

CALFPED

CYTCNGGRAANARNGCACA

RNBS-D non-TIR primer 3 (nT3)

CALFPED

CYTCNGGRAANARNGCGCA

Conserved GLPL primer 1 (1)

CGGLPLA

GCIARIGGIARICCICCRCA

Conserved GLPL primer 2 (2)

CGGLPLA

GCNARNGGNARNCCNCCACA

Conserved GLPL primer 3 (3)

CGGLPLA

GCNARNGGNARNCCNCCGCA

  1. Primers are based on previously reported consensus amino acid sequences [7], with the exception of the primer to the P-loop sequence GIGKTT, which was reported as the P-loop consensus sequence for the TIR-NBS-LRR group [9].