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Table 4 Primers used for amplification of NBS sequences

From: TIR-NBS-LRR genes are rare in monocots: evidence from diverse monocot orders

Specificity (abbreviation) Amino acid sequence Degenerate primer (5'-3')
Conserved P-loop/Kinase-1a (V) GVGKTT GGIGTIGGIAARACIAC
P-loop/Kinase-1a TIR (I) GIGKTT GGIATHGGIAARACIAC
RNBS-D TIR primer 1L (T1L) FLHIACFF RAARAARCAIGCDATRTGIARRAA
RNBS-D TIR primer 1 (T1) FLHIAC CAIGCDATRTGIARRAA
RNBS-D TIR primer 2 (T2) FLHIAC CANGCDATRTGAARRAA
RNBS-D TIR primer 3 (T3) FLHIAC CANGCDATRTGCARRAA
RNBS-D TIR primer 4 (T4) FLHIAC CANGCDATRTGGARRAA
RNBS-D TIR primer 5 (T5) FLHIAC CANGCDATRTGTARRAA
RNBS-D non-TIR primer 1 (nT1) CFLYCALFPED CYTCIGGRAAIARIGCRCARTAIARRAARC
RNBS-D non-TIR primer 2 (nT2) CALFPED CYTCNGGRAANARNGCACA
RNBS-D non-TIR primer 3 (nT3) CALFPED CYTCNGGRAANARNGCGCA
Conserved GLPL primer 1 (1) CGGLPLA GCIARIGGIARICCICCRCA
Conserved GLPL primer 2 (2) CGGLPLA GCNARNGGNARNCCNCCACA
Conserved GLPL primer 3 (3) CGGLPLA GCNARNGGNARNCCNCCGCA
  1. Primers are based on previously reported consensus amino acid sequences [7], with the exception of the primer to the P-loop sequence GIGKTT, which was reported as the P-loop consensus sequence for the TIR-NBS-LRR group [9].