Nucleotide sequence of clone C13 of the cDNAs library of D. antarctica and plasmid construct. A. The deduced amino acid sequence is shown in one-letter code above the nucleotide sequence. Asterisks indicate the stop signal. B. Recombinant plasmid vector. The SOD gene from D. antarctica was cloned in frame with the α-factor secretion signal peptide at the N-terminal and with the myc epitope and the 6xHis-tag at the C-terminal into the pGAPZαA vector resulting in the pGAPZαA-SOD.