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Figure 4 | BMC Research Notes

Figure 4

From: Cloning and constitutive expression of Deschampsia antarctica Cu/Zn superoxide dismutase in Pichia pastoris

Figure 4

Deglycosylation of recombinant SOD by treatment with PNGase F. Crude and purified supernatant (SN) samples from the SMD1168H-pGAPZαA-SOD strain were treated without (-) or with (+) 1000 U PNGase F. After the deglycosylation treatment the samples were analyzed by SDS-PAGE and immunoblotting using myc-specific (Myc) antisera. Mw, molecular weight marker (Fermentas).

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