Table 1 Purification of recombinant SOD expressed by Pichia pastoris
| Step |
Volume (ml) |
Protein (mg) |
Total activity (U) |
Specific activity (U/mg) |
Yield (%) |
|---|---|---|---|---|---|
| Supernatant | 45 | 11.25 | 900 | 80 | 100.0 |
| Concentrated (1/5) | 9 | 6.40 | 134 | 21 | 14.9 |
| ProBond | 8 | 0.26 | 113 | 440 | 12.5 |