Immunofluoresence analysis of larvae. Mixtures of mutant and control larvae of different sexes were used. Sex lethal (Tx red) is used to distinguish male and female nuclei. H3K4me3 is stained by FITC. About 50 such pairs of nuclei were observed and a representative image is shown. Acid extracted histones from larvae were used for western blotting. CG11033-UAS-RNAi larvae (no Gal4) were used as controls while larvae expressing act5c-Gal4 were used as knockdown mutants.