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Figure 2 | BMC Research Notes

Figure 2

From: Saliva as an alternative source of high yield canine genomic DNA for genotyping studies

Figure 2

Amplification of an 1.1-kb fragment using DNA isolated from saliva, buccal swab and blood samples. 100 ng of canine genomic DNA isolated using the Oragene ®•ANIMAL kit (An; lanes 1, 4, 7, 9, 11, 13, 15, 17, 19, 21, 24, 26, 28, 30 and 32), buccal swabs (Bu; lanes 2, 5, 8, 10, 12, 14, 16, 18, 20, 22, 25, 27, 29, 31 and 33) or blood (Bl; lanes 3, 6 and 23) were used for PCR amplification of an 1155-bp fragment in the coding region of the canine transferrin receptor gene. Reaction products were resolved by agarose gel electrophoresis and visualized by staining with SYBR® Green.

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