TY - JOUR AU - Rodrigues, Valnês S. AU - Breda, Ardala AU - Santos, Diógenes S. AU - Basso, Luiz A. PY - 2009 DA - 2009/11/16 TI - The conserved Lysine69 residue plays a catalytic role in Mycobacterium tuberculosis shikimate dehydrogenase JO - BMC Research Notes SP - 227 VL - 2 IS - 1 AB - The shikimate pathway is an attractive target for the development of antitubercular agents because it is essential in Mycobacterium tuberculosis, the causative agent of tuberculosis, but absent in humans. M. tuberculosis aroE-encoded shikimate dehydrogenase catalyzes the forth reaction in the shikimate pathway. Structural and functional studies indicate that Lysine69 may be involved in catalysis and/or substrate binding in M. tuberculosis shikimate dehydrogenase. Investigation of the kinetic properties of mutant enzymes can bring important insights about the role of amino acid residues for M. tuberculosis shikimate dehydrogenase. SN - 1756-0500 UR - https://doi.org/10.1186/1756-0500-2-227 DO - 10.1186/1756-0500-2-227 ID - Rodrigues2009 ER -