Quantitative analysis of cytokines. The amounts of several cytokines in media of PBMC cultures treated for 24 h with L4 in the presence of 1 ng/ml LPS (+LPS) or in the absence (-LPS) were determined. (A) IL-1β, (B) IL-6, (C) IL-10, (D) TNF-α, and (E) IL-8. The concentration of L4 in each assay is indicated. Assays were done in quadruplicate.