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Table 1 aplnra function is required for normal epiboly and elongation/extension movements

From: Zebrafish aplnra functions in epiboly

  

%

Age (normal developmental stage)

Apparent Developmental Stagea

A

B

C

D

Control

8 hpf (75% epiboly)

75% epiboly-bud

11

10

27

65

80

 

50%-75% epiboly

66

75

70

28

16

 

30%-50% epiboly

23

14

3

8

4

 

(n) =

(96)

(69)

(60)

(65)

(48)

14 hpf (10 somite)

10 somite stage+

22

3

10

18

87

 

6-10 somite stage

8

17

24

71

6

 

bud-6 somite stage

25

59

37

3

2

 

75% epiboly-bud

20

13

18

3

2

 

50% - 75% epiboly

10

6

8

3

2

 

30%-50% epiboly

15

2

3

2

1

 

(n) =

(61)

(57)

(62)

(65)

(46)

24 hpf (prim-5)

24 hpf+

7

2

13

66

88

 

18-24 hpf

48

3

27

19

9

 

bud-18 hpf

27

81

33

11

2

 

75% epiboly-bud

6

11

11

0

1

 

50% - 75% epiboly

4

3

11

3

0

 

30%-50% epiboly

7

0

6

2

0

 

(n) =

(67)

(63)

(64)

(64)

(45)

 

Ectopic Cell Massb

67

84

17

4

1

  1. Injection of MO2 at different concentrations results in different levels of developmental delay in terms of epiboly and elongation/extension. Injection of MO2 at later developmental stages (into yolk cell vegetal to embryo at 32-cell stage, partially restricting localisation of MO2 to the YSL) leads to similar, if slightly reduced, levels of delay as found in embryos injected at the 1-cell stage. This indicates that aplnra has critical functions primarily in the YSL. A = 1 mM MO2 injection at 32 cell stage (animal pole of the yolk), B = 1 mM MO2 injection at 1 cell stage, C = 0.50 mM MO2 injection at 1 cell stage, D = 0.25 mM MO2 injection at 1 cell stage, Control = 1 mM MOCont injection at 1 cell stage. Notes: a As determined by extent of epiboly or extension/elongation. b Ectopic cell mass located dorsally to the early tail-bud/origin of the yolk extension, depending on apparent developmental stage. Represented as % of the total number of embryos injected (24 hpf).