Skip to main content

Advertisement

Table 1 aplnra function is required for normal epiboly and elongation/extension movements

From: Zebrafish aplnra functions in epiboly

   %
Age (normal developmental stage) Apparent Developmental Stagea A B C D Control
8 hpf (75% epiboly) 75% epiboly-bud 11 10 27 65 80
  50%-75% epiboly 66 75 70 28 16
  30%-50% epiboly 23 14 3 8 4
  (n) = (96) (69) (60) (65) (48)
14 hpf (10 somite) 10 somite stage+ 22 3 10 18 87
  6-10 somite stage 8 17 24 71 6
  bud-6 somite stage 25 59 37 3 2
  75% epiboly-bud 20 13 18 3 2
  50% - 75% epiboly 10 6 8 3 2
  30%-50% epiboly 15 2 3 2 1
  (n) = (61) (57) (62) (65) (46)
24 hpf (prim-5) 24 hpf+ 7 2 13 66 88
  18-24 hpf 48 3 27 19 9
  bud-18 hpf 27 81 33 11 2
  75% epiboly-bud 6 11 11 0 1
  50% - 75% epiboly 4 3 11 3 0
  30%-50% epiboly 7 0 6 2 0
  (n) = (67) (63) (64) (64) (45)
  Ectopic Cell Massb 67 84 17 4 1
  1. Injection of MO2 at different concentrations results in different levels of developmental delay in terms of epiboly and elongation/extension. Injection of MO2 at later developmental stages (into yolk cell vegetal to embryo at 32-cell stage, partially restricting localisation of MO2 to the YSL) leads to similar, if slightly reduced, levels of delay as found in embryos injected at the 1-cell stage. This indicates that aplnra has critical functions primarily in the YSL. A = 1 mM MO2 injection at 32 cell stage (animal pole of the yolk), B = 1 mM MO2 injection at 1 cell stage, C = 0.50 mM MO2 injection at 1 cell stage, D = 0.25 mM MO2 injection at 1 cell stage, Control = 1 mM MOCont injection at 1 cell stage. Notes: a As determined by extent of epiboly or extension/elongation. b Ectopic cell mass located dorsally to the early tail-bud/origin of the yolk extension, depending on apparent developmental stage. Represented as % of the total number of embryos injected (24 hpf).