| | |
%
|
|---|
|
Age (normal developmental stage)
|
Apparent Developmental Stagea
|
A
|
B
|
C
|
D
|
Control
|
|---|
|
8 hpf (75% epiboly)
|
75% epiboly-bud
|
11
|
10
|
27
|
65
|
80
|
| |
50%-75% epiboly
|
66
|
75
|
70
|
28
|
16
|
| |
30%-50% epiboly
|
23
|
14
|
3
|
8
|
4
|
| |
(n) =
|
(96)
|
(69)
|
(60)
|
(65)
|
(48)
|
|
14 hpf (10 somite)
|
10 somite stage+
|
22
|
3
|
10
|
18
|
87
|
| |
6-10 somite stage
|
8
|
17
|
24
|
71
|
6
|
| |
bud-6 somite stage
|
25
|
59
|
37
|
3
|
2
|
| |
75% epiboly-bud
|
20
|
13
|
18
|
3
|
2
|
| |
50% - 75% epiboly
|
10
|
6
|
8
|
3
|
2
|
| |
30%-50% epiboly
|
15
|
2
|
3
|
2
|
1
|
| |
(n) =
|
(61)
|
(57)
|
(62)
|
(65)
|
(46)
|
|
24 hpf (prim-5)
|
24 hpf+
|
7
|
2
|
13
|
66
|
88
|
| |
18-24 hpf
|
48
|
3
|
27
|
19
|
9
|
| |
bud-18 hpf
|
27
|
81
|
33
|
11
|
2
|
| |
75% epiboly-bud
|
6
|
11
|
11
|
0
|
1
|
| |
50% - 75% epiboly
|
4
|
3
|
11
|
3
|
0
|
| |
30%-50% epiboly
|
7
|
0
|
6
|
2
|
0
|
| |
(n) =
|
(67)
|
(63)
|
(64)
|
(64)
|
(45)
|
| |
Ectopic Cell Massb
|
67
|
84
|
17
|
4
|
1
|
- Injection of MO2 at different concentrations results in different levels of developmental delay in terms of epiboly and elongation/extension. Injection of MO2 at later developmental stages (into yolk cell vegetal to embryo at 32-cell stage, partially restricting localisation of MO2 to the YSL) leads to similar, if slightly reduced, levels of delay as found in embryos injected at the 1-cell stage. This indicates that aplnra has critical functions primarily in the YSL. A = 1 mM MO2 injection at 32 cell stage (animal pole of the yolk), B = 1 mM MO2 injection at 1 cell stage, C = 0.50 mM MO2 injection at 1 cell stage, D = 0.25 mM MO2 injection at 1 cell stage, Control = 1 mM MOCont injection at 1 cell stage. Notes: a As determined by extent of epiboly or extension/elongation. b Ectopic cell mass located dorsally to the early tail-bud/origin of the yolk extension, depending on apparent developmental stage. Represented as % of the total number of embryos injected (24 hpf).
