TY - JOUR AU - Vermeulen, Joƫlle AU - Derveaux, Stefaan AU - Lefever, Steve AU - De Smet, Els AU - De Preter, Katleen AU - Yigit, Nurten AU - De Paepe, Anne AU - Pattyn, Filip AU - Speleman, Frank AU - Vandesompele, Jo PY - 2009 DA - 2009/11/25 TI - RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts JO - BMC Research Notes SP - 235 VL - 2 IS - 1 AB - The quantitative polymerase chain reaction (qPCR) is a widely utilized method for gene-expression analysis. However, insufficient material often compromises large-scale gene-expression studies. The aim of this study is to evaluate an RNA pre-amplification method to produce micrograms of cDNA as input for qPCR. SN - 1756-0500 UR - https://doi.org/10.1186/1756-0500-2-235 DO - 10.1186/1756-0500-2-235 ID - Vermeulen2009 ER -