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Table 1 Expression values of 6 reference genes using qPCR in purified versus non-purified pre-amplified samples

From: RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts

   UBC GAPDH RPL13A YWHAZ SDHA HPRT1 mean dCq 2^dCq
sample 1 mean Cq NP1 (n = 2) 25.47 23.73 19.05 22.31 29.82 25.71   
  mean Cq P1 (n = 2) 21.54 22.19 15.87 18.29 25.94 22.03   
  dCq 3.93 1.54 3.18 4.02 3.88 3.68 3.37 10.34
sample 2 mean Cq NP2 (n = 2) 23.71 23.03 18.12 21.81 30.14 24.86   
  mean Cq P2 (n = 2) 20.95 21.54 15.63 18.53 28.14 21.89   
  dCq 2.77 1.49 2.49 3.28 2.01 2.97 2.5 5.65
sample 3 mean Cq NP3 (n = 2) 24.22 23.03 18.63 20.94 30.17 25.82   
  mean Cq P3 (n = 2) 21.1 21.75 16.04 17.66 28.67 22.72   
  dCq 3.12 1.29 2.6 3.28 1.5 3.1 2.48 5.57
sample 4 mean Cq NP4 (n = 2) 22.96 23.08 18.54 21.74 30.66 25.62   
  mean Cq P4 (n = 2) 19.69 21.89 16.07 18.63 28.83 22.48   
  dCq 3.27 1.19 2.48 3.11 1.83 3.14 2.5 5.66
sample 5 mean Cq NP5 (n = 2) 23.21 22.72 17.96 20.84 28.17 25.37   
  mean Cq P5 (n = 2) 20.64 21.22 15.6 17.83 25.73 22.11   
  dCq 2.57 1.51 2.36 3.01 2.44 3.26 2.52 5.74
sample 6 mean Cq NP6 (n = 2) 24.62 23.48 18.56 22.22 28.84 25.71   
  mean Cq P6 (n = 2) 21.52 21.87 15.91 19.03 26.97 22.67   
  dCq 3.1 1.61 2.65 3.19 1.88 3.04 2.58 5.96
average          6.31 (95% CI: 4.89 - 8.14)
  1. Cq: quantification cycle
  2. dCq: difference in Cq or delta-Cq
  3. NP: non-purified after pre-amplification
  4. P: purified after pre-amplification
  5. 2^dCq designates the pre-amplifiable target ratio (purified/non-purified products)