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Table 1 Volumes of buffer required for protocol scale up according to culture vessel surface area.

From: Crude subcellular fractionation of cultured mammalian cell lines

Culture Vessel Surface Area (cm2) Buffer Volume (ml)
100 mm dish 55 5.8
60 mm dish 21 2.2
35 mm dish 8 0.84
6 well plate 9 0.95
12 well plate 3.8 0.4
24 well plate 2.0 0.2