Table 1 Volumes of buffer required for protocol scale up according to culture vessel surface area.
From: Crude subcellular fractionation of cultured mammalian cell lines
Culture Vessel | Surface Area (cm2) | Buffer Volume (ml) |
|---|---|---|
100 mm dish | 55 | 5.8 |
60 mm dish | 21 | 2.2 |
35 mm dish | 8 | 0.84 |
6 well plate | 9 | 0.95 |
12 well plate | 3.8 | 0.4 |
24 well plate | 2.0 | 0.2 |