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Table 2 Primer Sequences and Combinations used in PCR-SSP-typing for Interleukin-6 Promoter -597/-572/-174Haplotype

From: Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter -597/-572/-174Haplotypes

  Primermix Primer Sequence Product
Size [bp]
- 597
Genotype
- 527
Genotype
- 174
Genotype
Detected
major & (minor) Haplotypes
I AS2-F3a
AS8-R1g
5'-tgaagtaactgcacgaaatttgagga-3'
5'-tgcaatgtgacgtcctttagcatg-3'
473 A - C AGC
(ACC)
II AS1-F1g
AS8-R1g
5'-aagtaactgcacgaaatttgaggg-3'
5'-tgcaatgtgacgtcctttagcatg-3'
471 G - C GGC
(GCC)
III AS3-F3g
AS7-R1c
5'-grtggccaggcagttctacaacagccg-3'
5'-tgcaatgtgacgtcctttagcatc-3'
449 - G G GGG
(AGG)
IV AS4-F3c
AS7-R1c
5'-grtggccaggcagttctacaacagccc-3'
5'-tgcaatgtgacgtcctttagcatc-3'
449 - C G GCG
(ACG)
  β-globF4
β-globR5
5'-gcttaccaagctgtgattcc-3'
5'-aaggtgcccttgaggttgtc-3'
731