Selection of reference genes for quantitative real-time PCR in equine in vivo and fresh and frozen-thawed in vitro blastocysts

Table 1 Primers

Gene	GenBank accession number	Sequence	Amplicon size (bp)	Ta (°C)	RTprimerDB ID	C_q range
ACTB	AF035774	CCAGCACGATGAAGATCAAG GTGGACAATGAGGCCAGAAT	88	60	7848	16.3-23.8
GAPDH	XM_001496020.1	CAGAACATCATCCCTGCTTC ATGCCTGCTTCACCACCTTC	187	59	7849	14.2-24.5
H2A/I	XM_001497311.2	ATATTCAGGCCGTGCTGCT TTTGGGTTTCAAAGCGTTTC	105	60	*	23.5-37.3
HPRT1	AY372182	GGCAAAACAATGCAAACCTT CAAGGGCATATCCTACGACAA	163	57	7850	17.6-25.8
RPL32	XM_001492042.2	AGCCATCTACTCGGCGTCA TCCAATGCCTCTGGGTTTC	149	60	*	16.8-25.9
SDHA	XM_001490889	TCCATCGCATAAGAGCAAAG GGTGGAACTGAACGAACTCC	159	59	7851	20.7-33.3
TUBA4A	XM_001491910.2	GCCCTACAACTCCATCCTGA ATGGCTTCATTGTCCACCA	78	60	*	16.6-27.9
UBC	AF506969	GCAAGACCATCACCCTGGA CTAACAGCCACCCCTGAGAC	206	60	7874	15.5-23.7

For each reference gene, the NCBI GenBank accession number, the sequence of both forward and reverse primer, the size of the amplicon and the optimal primer annealing temperature are listed. The RTPrimerDB ID http://rtprimerdb.org/ is also reported, except for the primers for which there is not yet an official reference sequence available in the database (*).

ISSN: 1756-0500