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Figure 2 | BMC Research Notes

Figure 2

From: Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays

Figure 2

Flow diagram describing computational steps of the normalization algorithm. Input data are median background subtracted median spot pixel intensities. In the second step, control channel spot intensities are adjusted to the level of probe channel intensities. Next, negative values are replaced by a small number to avoid taking negative logarithm. Fourth, probe channel spot values are adjusted by multiplying the probe spot value by natural logarithm of the corresponding ratio of probe and control spot values. Finally, if median of replicate spots is negative, the difference is added to the spot values to set the median to the minimum of zero.

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