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Table 3 Average M2 frequencies of chlorophyll seedling mutants (%) after different EMS treatments

From: TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1

Classificationa) Phenotype Fb) EMS treatment
    20 mM1) 25 mM2) 30 mM3) 35 mM4) 40 mM5) 50 mM6)
Unicolour albina 11.8*** 1.5 2.3 3.4 4.3 6.6 7.0
Unicolour xantha 0.61 0.2 0.6 1.2 0.4 1.5 -
Unicolour viridis 1.10 0.1 0.4 0.6 1.3 0.5 -
Bicolour viridoalbina 1.62 0.4 0.2 0.4 0.8 0.5 -
Bicolour striata 1.08 0.15 0.1 0.3 0.9 0.5 -
  1. Relative frequencies were calculated as mutations per 100 germinated M2 plants according to [20]. Number of analysed individuals: 1) 17,616; 2) 13,881; 3) 20,820; 4) 14,190; 5) 1,270; 6) 719. Chlorophyll mutant rates other than albina observed among M2seedlings obtained from treatments higher than 40 mM were excluded from statistical considerations because the generally low germination rate of those mutants was insufficient.
  2. a) Chlorophyll seedling mutant classification according to [20].
  3. b) The one factorial analysis of variance (ANOVA) tested the variability within each EMS concentration and between all concentrations. ANOVA: F = Fisher's exact test: *: Significance at p ≤ 0.05; ** Significance at p ≤ 0.01; *** Significance at p ≤ 0.001. A test of linearity was performed to confirm the required normality of the single data set (data not shown) [63].
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