Table 1 Summary of technical performance of four neo-epitope specific immunoassays
Parameter | NITEGE373 | G1-NITEGE373 | 374ARGSV | 374ARGSV-G2 |
|---|---|---|---|---|
Measuring range (ng/ml) | 12.3 - 1000 | 120 - 4000 | 0.4 - 20 | 16.5 - 1000 |
Lower detection limit (ng/ml) | 4.1 | 40.1 | 0.1 | 5.5 |
Intraassay variation (CV, %) | 4.2 - 9.6 | 4.0 - 6.0 | 7.4 - 11.2 | 2.0 - 3.5 |
Interassay variation (CV, %) | 7.1 - 13.2 | 7.5 - 13.4 | 9.9 - 12.4 | 8.9 - 10.3 |
Dilution recovery (mean; range,%) | 103.6; 88.9 - 118.2 | 106.6; 94.6 - 119.2 | 101.4; 89.3 - 117.1 | 93.4; 82.7 - 103.5 |
Specificity (%) | PLPRNITEGE; 100 PLPRNITEGEAR; <0.1 ARGSVILTVK; <0.1 | ARGSVILTVK; 100 GEARGSVILTVK; <0.1 PLPRNITEGE; <0.1 |