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Table 1 Summary of technical performance of four neo-epitope specific immunoassays

From: Suppression of MMP activity in bovine cartilage explants cultures has little if any effect on the release of aggrecanase-derived aggrecan fragments

Parameter NITEGE373 G1-NITEGE373 374ARGSV 374ARGSV-G2
Measuring range (ng/ml) 12.3 - 1000 120 - 4000 0.4 - 20 16.5 - 1000
Lower detection limit (ng/ml) 4.1 40.1 0.1 5.5
Intraassay variation (CV, %) 4.2 - 9.6 4.0 - 6.0 7.4 - 11.2 2.0 - 3.5
Interassay variation (CV, %) 7.1 - 13.2 7.5 - 13.4 9.9 - 12.4 8.9 - 10.3
Dilution recovery (mean; range,%) 103.6;
88.9 - 118.2
106.6;
94.6 - 119.2
101.4;
89.3 - 117.1
93.4;
82.7 - 103.5
Specificity (%) PLPRNITEGE; 100
PLPRNITEGEAR; <0.1
ARGSVILTVK; <0.1
  ARGSVILTVK; 100
GEARGSVILTVK; <0.1
PLPRNITEGE; <0.1