Figure 4

Examples of poor quality raw AFLP electropherograms. In most cases, the only solution is to run PCR again. A. An example of the AFLP electropherogram in which the AFLP data (as indicated by the blue line) is too low and is located below the size standard (the red line). The peak intensity is too low and does not permit confident scoring. B. In this example, the peak intensity forms a hill and should not be scored. C. During this run, as can be seen by the electropherogram, the analysis stopped working around 6000 frames. D. The peak intensity is too high and has saturated the ABI 3100. The saturation point for the ABI 3100 is 8000 relative fluorescence units (rfu). The peaks that are squared off at the 8000 rfu point cannot be confidently scored.