Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Table 1 Adapter and primer sequences used in AFLP.

Adapter Sequence
Eco RI-Adapter	5'-CTCGTAGACTGCGTACCAATTC-3' 3'CATCTGACGCATGGTTAAG-5'
Mse I-Adapter	5'-GACGATGAGTCCTGAGTAA-3' 3'-TACTCAGGACTCATT-5'
Snails	*Eco* RI Sequence (5'-3')	*Mse* l Sequence (5'-3')
Preselective Primer 1	CTCGTAGACTGCGTACCAATTCGACCA	GACGATGAGTCCTGAGTAAGACCG
Preselective Primer 2	CTCGTAGACTGCGTACCAATTCGACCA	GACGATGAGTCCTGAGTAAGACGC
Preselective Primer 3	CTCGTAGACTGCGTACCAATTCGACCT	GACGATGAGTCCTGAGTAAGACCG
Preselective Primer 4	CTCGTAGACTGCGTACCAATTCGACCT	GACGATGAGTCCTGAGTAAGACGC
Selective Primer 1	CTCGTAGACTGCGTACCAATTCGACCAA	GACGATGAGTCCTGAGTAAGACCG
Selective Primer 2	CTCGTAGACTGCGTACCAATTCGACCAA	GACGATGAGTCCTGAGTAAGACGC
Selective Primer 3	CTCGTAGACTGCGTACCAATTCGACCTG	GACGATGAGTCCTGAGTAAGACCG
Selective Primer 4	CTCGTAGACTGCGTACCAATTCGACCTG	GACGATGAGTCCTGAGTAAGACGC
Salamanders
Preselective Primer 1	CTCGTAGACTGCGTACCAATTCGACCA	GACGATGAGTCCTGAGTAAGACGA
Preselective Primer 2	CTCGTAGACTGCGTACCAATTCGACCA	GACGATGAGTCCTGAGTAAGACGC
Preselective Primer 3	CTCGTAGACTGCGTACCAATTCGACCA	GACGATGAGTCCTGAGTAAGACCG
Preselective Primer 4	CTCGTAGACTGCGTACCAATTCGACAC *	GACGATGAGTCCTGAGTAAGACCA *
Selective Primer 1	CTCGTAGACTGCGTACCAATTCGACCAA	GACGATGAGTCCTGAGTAAGACCA
Selective Primer 2	CTCGTAGACTGCGTACCAATTCGACCAA	GACGATGAGTCCTGAGTAAGACGC
Selective Primer 3	CTCGTAGACTGCGTACCAATTCGACCAA	GACGATGAGTCCTGAGTAAGACCG
Selective Primer 4	CTCGTAGACTGCGTACCAATTCGACACC *	GACGATGAGTCCTGAGTAAGACCA *

Adapter and preselective and selective primer sequences for the rare cutter, Eco RI and the frequent cutter, Mse I. Black text indicates the core sequence of either of the adapter, Eco RI or Mse I sequences. Black Bold text indicates the restriction portion of the adapter sequence. Black Bold Italicized text indicates the preselective or selective primer sequence. The * indicates a primer combination used in the salamander portion of the study, but the results are not presented here.

ISSN: 1756-0500