Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Table 2 Cocktails used for preselective PCR in AFLP.

Preselective Cocktail
Reagent	Quantity (μl)
15 pmol Preselective Eco RI Primer	1.2
15 pmol Preselective Mse l Primer	1.2
10 mM dNTP	4.0
Formamide	1.0
25 mM MgCl₂	2.5
H₂O	24.8
10× PCR Buffer	10.0
Taq DNA Polymerase	0.5
Total	45.2*
*Add 40 μl of the preselective cocktail to 10 μl diluted restriction-ligation product. The remaining 5.2 μl allows for pipetting error.
Reaction cocktails for preselective PCR amplifications of the ligated DNA.

ISSN: 1756-0500