Preselective Cocktail | Â |
---|---|
Reagent | Quantity (μl) |
15 pmol Preselective Eco RI Primer | 1.2 |
15 pmol Preselective Mse l Primer | 1.2 |
10 mM dNTP | 4.0 |
Formamide | 1.0 |
25 mM MgCl2 | 2.5 |
H2O | 24.8 |
10× PCR Buffer | 10.0 |
Taq DNA Polymerase | 0.5 |
Total | 45.2* |
*Add 40 μl of the preselective cocktail to 10 μl diluted restriction-ligation product. The remaining 5.2 μl allows for pipetting error. |  |
Reaction cocktails for preselective PCR amplifications of the ligated DNA. | Â |