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Table 2 Cocktails used for preselective PCR in AFLP.

From: Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Preselective Cocktail  
Reagent Quantity (μl)
15 pmol Preselective Eco RI Primer 1.2
15 pmol Preselective Mse l Primer 1.2
10 mM dNTP 4.0
Formamide 1.0
25 mM MgCl2 2.5
H2O 24.8
10× PCR Buffer 10.0
Taq DNA Polymerase 0.5
Total 45.2*
*Add 40 μl of the preselective cocktail to 10 μl diluted restriction-ligation product. The remaining 5.2 μl allows for pipetting error.  
Reaction cocktails for preselective PCR amplifications of the ligated DNA.