Skip to main content

Table 5 Thermocycler conditions for selective PCR.

From: Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Selective Cycles
Step Temperature Duration Number of Cycles
Initial Denaturation 94°C 120 sec 1
Denaturation 94°C 50 sec  
Annealing 56°C 60 sec 2
Extension 72°C 60 sec  
Denaturation 94°C 50 sec  
Annealing 56°C 60 sec 20
Extension 72°C 120 sec  
Final Extension 72°C 10 min 1