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Table 3 Summary of phenotypic characteristics of the field Brucella spp. from Zimbabwea

From: Characterization of some Brucella species from Zimbabwe by biochemical profiling and AMOS-PCR

 

Growth characteristics

Monospecific Sera

Phages at RTD

AMOS-PCR

Interpretation

Isolate No.

CO2Dependent

H2S

BF

TH

A

M

Tb

BK2

Fi

Iz1

R/C

Size of DNA detected

 

B1, B6, B7, B14, B16, B21

-

+

+

-

+

-

CL

CL

CL

CL

NL

498 bp

B. abortus 1

B15

-

+

-

-

+

-

CL

CL

CL

CL

CL

498 bp

B. abortus 2

B10

-

-

 

+

-

+

NL

PL

NL

NL

NL

731 bp

B. melitensis 1

B4, B5, B8, B9, B12

+

+

+

-

+

-

CL

CL

CL

CL

NL

498 bp

B. abortus 1

B20

+

+

-

-

+

-

CL

CL

CL

CL

NL

498 bp

B. abortus 2

  1. a All tests carried out by the reference laboratory (VLA), Weybridge, UK.
  2. Isolate No. = Isolate identification
  3. DNA: Test by the AMOS PCR
  4. BF = Basic fuchsin at 20 μl/ml (1/50,000 w/v)
  5. TH = Thionin at 20 μl/ml (1/50,000 w/v)
  6. Phages: Tb = Tbilisi, BK2 = Berkeley type 2, Fi = Firenze, Iz1 = Izatnagar, R/C = phage lytic for non-smooth species of Brucella
  7. CL = Confluent Lysis
  8. PL = Partial lysis
  9. NL = No lysis
  10. RTD = Routine test dilution
  11. + = positive (yes)
  12. - negative (no)
  13. bp = base pairs