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Table 3 Summary of phenotypic characteristics of the field Brucella spp. from Zimbabwea

From: Characterization of some Brucella species from Zimbabwe by biochemical profiling and AMOS-PCR

  Growth characteristics Monospecific Sera Phages at RTD AMOS-PCR Interpretation
Isolate No. CO2Dependent H2S BF TH A M Tb BK2 Fi Iz1 R/C Size of DNA detected  
B1, B6, B7, B14, B16, B21 - + + - + - CL CL CL CL NL 498 bp B. abortus 1
B15 - + - - + - CL CL CL CL CL 498 bp B. abortus 2
B10 - -   + - + NL PL NL NL NL 731 bp B. melitensis 1
B4, B5, B8, B9, B12 + + + - + - CL CL CL CL NL 498 bp B. abortus 1
B20 + + - - + - CL CL CL CL NL 498 bp B. abortus 2
  1. a All tests carried out by the reference laboratory (VLA), Weybridge, UK.
  2. Isolate No. = Isolate identification
  3. DNA: Test by the AMOS PCR
  4. BF = Basic fuchsin at 20 μl/ml (1/50,000 w/v)
  5. TH = Thionin at 20 μl/ml (1/50,000 w/v)
  6. Phages: Tb = Tbilisi, BK2 = Berkeley type 2, Fi = Firenze, Iz1 = Izatnagar, R/C = phage lytic for non-smooth species of Brucella
  7. CL = Confluent Lysis
  8. PL = Partial lysis
  9. NL = No lysis
  10. RTD = Routine test dilution
  11. + = positive (yes)
  12. - negative (no)
  13. bp = base pairs