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Table 1 Names, function, database ID and annealing temperature (Ta) of the tested primer sets

From: Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis

Abbreviation Gene Function Accession Number Ta [°C]
Actb-1 actin, beta cytoskeletal structural protein 2848# 60°C
Actb-2 actin, beta involved in cell motility, structure and integrity ensmusg00000029580+ 55°C
Actg1 actin, gamma, cytoplasmic1 Cytoskeletal structural protein ensmusg00000062825+ 55°C
B2 m-1 beta-2 microglobulin cytoskeletal protein involved in cell locomotion 3584# 60°C
B2 m-2 beta-2 microglobulin cytoskeletal protein involved in cell locomotion ensmusg00000060802+ 55°C
Canx calnexin protein folding and quality control in the endoplasmic reticulum ensmusg00000020368+ 55°C
Gapdh glyceraldehyde-3-phosphate dehydrogenase carbohydrate metabolism 3244# 60°C
Hprt1 hypoxanthine guanine phosphoribosyl transferase 1 metabolic salvage of purines in mammals 50# 55°C
Pgk1 phosphoglycerate kinase 1 transferase enzyme in the glycolysis ensmusg00000062070+ 55°C
Sdha succinate dehydrogenase complex, subunit A tricarboxylic acid cycle ensmusg00000021577+ 55°C
Rpl13a ribosomal protein L13A member of ribosome protein enst00000270634+ 55°C
Rplp0 ribosomal protein, large, P0 member of ribosome protein 2861# 60°C
Rps18 ribosomal protein S18 member of ribosome protein ensmusg00000008668+ 55°C
Tuba1a tubulin, alpha 1A structural protein 1484# 58°C
Ubc Ubiquitin C protein degradation ensmusg00000008348+ 55°C
  1. The eight reference genes that performed well in A. flavicollis liver samples are marked in bold.
  2. # RTPrimerDB: http://medgen.ugent.be/rtprimerdb
  3. + Ensembl Project: http://www.ensembl.org/index.html; Primer of Gene Normalization Panel