Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis

Table 1 Names, function, database ID and annealing temperature (Ta) of the tested primer sets

Abbreviation	Gene	Function	Accession Number	T_a [°C]
Actb-1	actin, beta	cytoskeletal structural protein	2848^#	60°C
*Actb-2*	actin, beta	involved in cell motility, structure and integrity	ensmusg00000029580⁺	55°C
*Actg1*	actin, gamma, cytoplasmic1	Cytoskeletal structural protein	ensmusg00000062825⁺	55°C
B2 m-1	beta-2 microglobulin	cytoskeletal protein involved in cell locomotion	3584^#	60°C
B2 m-2	beta-2 microglobulin	cytoskeletal protein involved in cell locomotion	ensmusg00000060802⁺	55°C
*Canx*	calnexin	protein folding and quality control in the endoplasmic reticulum	ensmusg00000020368⁺	55°C
Gapdh	glyceraldehyde-3-phosphate dehydrogenase	carbohydrate metabolism	3244^#	60°C
Hprt1	hypoxanthine guanine phosphoribosyl transferase 1	metabolic salvage of purines in mammals	50^#	55°C
*Pgk1*	phosphoglycerate kinase 1	transferase enzyme in the glycolysis	ensmusg00000062070⁺	55°C
*Sdha*	succinate dehydrogenase complex, subunit A	tricarboxylic acid cycle	ensmusg00000021577⁺	55°C
*Rpl13a*	ribosomal protein L13A	member of ribosome protein	enst00000270634⁺	55°C
Rplp0	ribosomal protein, large, P0	member of ribosome protein	2861^#	60°C
*Rps18*	ribosomal protein S18	member of ribosome protein	ensmusg00000008668⁺	55°C
Tuba1a	tubulin, alpha 1A	structural protein	1484^#	58°C
*Ubc*	Ubiquitin C	protein degradation	ensmusg00000008348⁺	55°C

The eight reference genes that performed well in A. flavicollis liver samples are marked in bold.
^# RTPrimerDB: http://medgen.ugent.be/rtprimerdb
⁺ Ensembl Project: http://www.ensembl.org/index.html; Primer of Gene Normalization Panel

ISSN: 1756-0500