Figure 1
From: The protein Nα-terminal acetyltransferase hNaa10p (hArd1) is phosphorylated in HEK293 cells
Detection of the hNaa10p protein by 2D-SDS PAGE. 80 μg of purified proteins from HEK293 cells were applied to both (A) and (B), and 50 μg proteins to (C). Molecular-size markers are indicated to the left. (A) A 18 cm NL IPG 3–10 was used in the first dimension, followed by SDS-PAGE in the second dimension. Proteins were visualized by using SYPRO-Ruby staining. (B) A 18 cm NL IPG 3–10 was used in the first dimension, followed by SDS-PAGE in the second dimension. Proteins were transferred to a nitrocellulose membrane and immunostained using anti-hNaa10p. (C) A 13 cm IPG 4–7 was used in the first dimension followed by SDS PAGE in the second dimension. Proteins were transferred to a nitrocellulose membrane and immunostained using anti-hNaa10p.