Figure 4
From: The protein Nα-terminal acetyltransferase hNaa10p (hArd1) is phosphorylated in HEK293 cells
hNaa10p in apoptotic and arsenic treated cells. 50 μg protein was analyzed by 2D-SDS PAGE using 13 cm IPG pH 4–7 for IEF. hNaa10p was detected by anti-hNaa10p. The molecular size markers are indicated to the left. (A) Control cells harvested after 3 days of culturing, (B) apoptotic cells, starved and harvested after 17 days, (C) cells were stimulated with 0.1 mM/16 hours arsenic and (D) cells were stimulated with 0.3 mM/16 hours arsenic.