hNaa10p in confluent and active cells. 50 μg proteins of each sample resuspended in rehydration buffer and analyzed by 2D-SDS PAGE using 13 cm IPG pH 4–7 for IEF. hNaa10p was detected by immunoblotting using anti-hNaa10p. The molecular size markers are indicated to the left. (A) Resting cells were grown for near 6 days without medium change. (B) Active cells were splitted into fresh medium 16 hours before harvesting.