Characterization of copy numbers of 16S rDNA and 16S rRNA of Candidatus Liberibacter asiaticus and the implication in detection in planta using quantitative PCR

Table 1 Comparison of copy numbers of 16S rDNA, beta-operon DNA, 16S rRNA, and beta-operon RNA

Copy number	Average	SD
beta-operon DNA	130316.70	17022.84
16S DNA	410145.75	51623.13
beta-operon RNA	1517.15	972.17
16S RNA	3211824.58	1899947.61
16S DNA/beta-operon DNA	3.15	0.11
16S RNA/16S DNA	7.83	4.12
beta-operon DNA/beta-operon RNA	85.90	71.93

Each citrus sample was divided into two Eppendorf tubes with 0.1 g each for DNA or RNA extraction and dissolved into 100 μl of water, respectively. 1 μl of DNA or RNA samples was used in either QPCR or QRT-PCR assays respectively. Totally, eight samples were used for calculation.

ISSN: 1756-0500