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Table 2 Comparison of QRT-PCR and QPCR assays

From: Characterization of copy numbers of 16S rDNA and 16S rRNA of Candidatus Liberibacter asiaticus and the implication in detection in planta using quantitative PCR

Dilution Ct value SD Ct value change SD
QPCR     
10 23.87 0.02   
102 27.16 0.17 3.29 0.17
103 30.47 0.31 3.30 0.48
104 33.93 0.20 3.46 0.11
QRT-PCR     
10 20.60 0.01   
102 23.77 0.08 3.17 0.08
103 26.82 0.14 3.06 0.18
104 29.53 0.12 2.70 0.12
105 32.99 0.76 3.47 0.80
  1. Each citrus sample was divided into two Eppendorf tubes with 0.1 g each for DNA or RNA extraction and dissolved into 100 μl of water, respectively. 1 μl of DNA or RNA samples was used in either QPCR or QRT-PCR assays respectively. Each value is the mean of three replicates.